Journal: Molecules

Article Title: Photoactivatable Caged Prodrugs of VEGFR-2 Kinase Inhibitors

doi: 10.3390/molecules21050570

Figure Lengend Snippet: Antiproliferative activity of tested compounds on VEGFR-2 dependent PC-3 cells. ( a ) Dose-response curves of active inhibitors ( 1 and 3 ) and their corresponding caged compounds ( 4 and 5 ) were determined without UV irradiation. Cell growth was measured 48 h after incubation with the compounds. The caged derivatives do not exhibit cytotoxic activity: the TGI mark is not reached even at high concentrations; ( b ) Cells were incubated for 1 h with the compounds and then irradiated at 365 nm (1.1 kW/m²) for 5 min. Cell growth was determined 48 h after incubation with the compounds. After irradiation, the caged derivatives show similar ( 5 ) or even left shifted ( 4 ) dose-response curves as that of active compounds. GI50 = 50% growth inhibition; TGI = total growth inhibition; LC 50 = 50% lethal concentration; n = 4. Error bars represent standard deviation. The effect of UV irradiation on the PC-3 cells is shown in .

Article Snippet: PC-3 cells were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: Activity Assay, Irradiation, Incubation, Inhibition, Concentration Assay, Standard Deviation

Journal: Molecules

Article Title: Photoactivatable Caged Prodrugs of VEGFR-2 Kinase Inhibitors

doi: 10.3390/molecules21050570

Figure Lengend Snippet: Antiproliferative activity of tested compounds in cellular growth assays with PC-3 cells, without and after irradiation at 365 nm (1.1 kW/m 2 ). The GI 50 values (50% cellular growth inhibition) are presented. “Not reached”: The tested compound did not inhibit the cell growth to 50%.

Article Snippet: PC-3 cells were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: Activity Assay, Irradiation, Inhibition

Journal: Molecules

Article Title: Photoactivatable Caged Prodrugs of VEGFR-2 Kinase Inhibitors

doi: 10.3390/molecules21050570

Figure Lengend Snippet: Microscopic images of stained PC-3 cells. The investigated compounds 3 and 5 are shown in green. Both compounds were applied at 10 µM concentrations. The cell nuclei were counterstained with 1 µg/mL DAPI and are marked blue. The dashed line represents 20.3 µm. ( a ) Staining with carbazole 3 ; ( b ) Staining with caged compound 5 .

Article Snippet: PC-3 cells were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: Staining

Journal: Heliyon

Article Title: Exploring the therapeutic potential of triterpenoid saponins from Gymnema sylvestre : Mechanistic insights into hepatoprotection, immunomodulation, anticancer activities, molecular docking, and pharmacokinetics

doi: 10.1016/j.heliyon.2024.e40850

Figure Lengend Snippet: Effect of triterpenoid saponin extract of G. sylvestre on cell viability of (a) COLO205 (Human Colon Cancer) and PC3 (Human prostate carcinoma) cells (b) cell viability of SKOV3 (Human ovarian cancer) and B16F10 (Mouse Melanoma) cell lines. The data is represented in the form of a bar graph and plotted using means ± SD of 3 individual experiments. Con: Control; GST: Triterpenoid saponin extract of G. sylvestre .

Article Snippet: All cancer cell lines, including COLO205 (Human colon cancer), PC3 (Human prostate carcinoma), SKOV3 (Human ovarian carcinoma), B16F10 (Mouse melanoma), MCF-7, and MDA-MB-231 as well as the normal CHO and HUVEC cell lines, were obtained from the National Centre for Cell Science (NCCS) in Pune, India.

Techniques: Control